Advanced Molecular Biology

Principles of gene amplification.

Principles of gene amplification; Polymerase chain reaction; Multiplex PCR; DNA and RNA extraction and purification; Expression vectors; promoters; plasmids and clones; Principles of plasmid ligation; Restriction digestion analysis; Principles of microarray and DNA chip analysis; Sequencing; Hybridization techniques; Gene transfer methods (electroporation; protoplast fusion); Gene addition and subtraction; Gene regulation; Genetic mapping; Gene and DNA markers: Restriction fragment length polymorphism (RFLP); Short tandem repeats or microsatellites (STR); Single nucleotide polymorphism (SNP); Expressed sequence tags (EST); Genetic fingerprinting and footprinting; Methods of DNA cloning and protein expression; DNA analysis in agriculture; medicine; forensic science and archeology