BTE 807
Research Techniques in Biotechnology
3
Course Description
RT-PCT.
Course Outline
RT-PCT; Real-Time PCR; Multiplex PCR; Vertical gel electrophoresis for proteins; Horizontal gel electrophoresis for nucleic acids; Northern and Southern blot analysis for nucleic acids; Gel-shift and gel retardation of DNA-protein complex analysis; Western blot analysis for proteins ELISA; Transformation; transfection and cloning; Rapid amplification of DNA ends (RACE); Cell and tissue culture; Mass Spectrometry (MALDI-TOF; etc.); Chromatographic analysis; Microarray and DNA chip analysis of transcriptomes and proteoms