Medical Bacteriology

At the end of this course, students should be able to: 1. identify basic safety procedures in the microbiology laboratory; 2. demonstrate staining techniques and use of light microscope; 3. discuss sterilisation and disinfection techniques; 4. explain cultivation of micro-organisms; 5. demonstrate biochemicals testing; 6. conduct slide agglutination tests; and 7. demonstrate sensitivity testing.

Practical bacteriology. Basic safety procedures in the microbiology laboratory. Staining techniques. Gram’s reaction, Ziehl-Nielsen reaction and spore staining reaction. Use of light microscope. Sterilisation and disinfection techniques. Cultivation of micro-organisms. Biochemicals testing. Slide agglutination tests. Sensitivity testing. MIC 403: Medical Mycology (1 Unit; C: LH 15) Learning Outcomes At the end of this course, students should be able to: 1. define superficial infections; 2. describe subcutaneous infections; 3. define cutaneous infections; 4. explain systemic infections; 5. describe opportunistic infections; 6. demonstrate skin scrapings for the diagnosis of superficial dermatomycoses; 7. identify fungal morphology in microscopy and on Sabourauds agar; 8. conduct rapid diagnostic tests; and 9. explain molecular diagnosis, PCR, ELISA. Course Contents Fungal infections, superficial, subcutaneous, cutaneous, systemic, opportunistic. Practical mycology, skin scrapings for the diagnosis of superficial dermatomycoses. Fungal morphology in microscopy and on Sabourauds agar. Rapid diagnostic tests. Molecular diagnosis, PCR, ELISA.