Laboratory Instrumentation & Techniques

At the end of this course, students should be able to: 1. describe the principles instrumentation; 2. explain care of basic equipment; 3. describe the theory and practice of some common Analytical techniques; and 4. discuss automation used in Medical Laboratory Science.

Instrument aspects of qualitative and quantitative analysis – theory and practise of some common analytical techniques: colourimetry, spectroflorimetry flame photometry, conductometry, polarography, and many others. Osmometry, Rephelometry, Turbidimetry, pH Measurement by ion specific electrodes – Separation techniques including electrophoresis, - paper, cellulose acetate, Agar gel, starch and polyacrylamide gel, Isoelectric focusing, Isotaphoresis, Chromatograpy – paper, Thin Layer Chromatography, Gas Liquid Chromatography, Ion exchange, gel filtration, molecular sieves; Dialysis filtration, solvent extraction, Centrifugation – Ultracentrifugation. Immuno-electrophoretic techniques, Radioimmunoassay, Competitive protein binding, Isotope dilution techniques, Enzyme Immuno Assays, Receptor Assays, Automation, Micro and Ultra micro Analysis. Practical based on the above topics. Theory and practice of some common Analytical techniques including tissue processing, Microscopy and other basic Microbiological Equipment, Principles and working of haematological Equipment, other applied techniques in the Medical Laboratory with emphasis on general Medical Laboratory Instrumentation. Practical exercises on the above topics.