Enzymes, Coenzymes and Bioenergetics

At the end of this course, students should be able to: 1. define enzymes and enumerate functions, nature and properties of enzymes; 2. describe kinetics of enzyme action; 3. enumerate allosteric effects; 4. explain enzyme assay in clinical medicine and immobilised enzymes; 5. identify hybridoma technology and monoclonal antibodies in medicine and biological research; 6. discuss the role of electron transport chain and oxidative phosphorylation in the generation of ATP, theories and models proposed for ATP generation; and 7. apply biochemical knowledge in the clinic.

Introduction to enzymes. Properties and functions, nature and properties of enzymes. Kinetics of enzyme action, allosteric effects. Enzyme assay in clinical medicine, immobilised enzymes, hybridoma technology and monoclonal antibodies in medicine and biological research. The role of electron transport chain and oxidative phosphorylation in the generation of ATP, theories and models proposed for ATP generation.